Journal: Discover Oncology

Article Title: Novel N 6 -methyladenosine (m 6 A) writer METTL16 promotes the cervical cancer tumorigenesis by targeting FTH1-dependent ferroptosis

doi: 10.1007/s12672-026-04403-8

Figure Lengend Snippet: FTH1 acted as the target of METTL16. A The immunofluorescence assay showed the subcellular localization of METTL16 and FTH1. B The binding motif sites of METTL16 for its target genes (GGAAC). C The FTH1 level in cervical cancer cells was test by the RT-PCR. D RNA immunoprecipitation (RIP) assay was conducted for the binding within METTL16 and FTH1 in HeLa cells. E RIP assay using anti-m 6 A conducted for the m 6 A modification on FTH1 mRNA. * p < 0.05, ** p < 0.01

Article Snippet: Cervical cancer cells were lysed by immunoprecipitation buffer (protease, phosphatase inhibitors, MCE) containing magnetic beads conjugated to specific antibodies (anti-IGF2BP2, anti-METTL16, anti-m 6 A).

Techniques: Immunofluorescence, Binding Assay, Reverse Transcription Polymerase Chain Reaction, RNA Immunoprecipitation, Modification

Journal: Discover Oncology

Article Title: Novel N 6 -methyladenosine (m 6 A) writer METTL16 promotes the cervical cancer tumorigenesis by targeting FTH1-dependent ferroptosis

doi: 10.1007/s12672-026-04403-8

Figure Lengend Snippet: IGF2BP2 promoted the stability of FTH1 mRNA. A The immunofluorescent staining showed the subcellular localization of IGF2BP2 and FTH1 in HeLa cells. B The positive correlation within IGF2BP2 and FTH1 in clinical samples ( http://gepia.cancer-pku.cn/index.html ). C RNA immunoprecipitation (RIP) assay showed the immunoprecipitated FTH1 mRNA by anti-IGF2BP2 antibody. D RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with IGF2BP2 overexpression. E RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with METTL16 silencing (sh-METTL16-1, sh-METTL16-2). F RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with IGF2BP2 silencing (si-IGF2BP2) and METTL16 overexpression. * p < 0.05, ** p < 0.01

Article Snippet: Cervical cancer cells were lysed by immunoprecipitation buffer (protease, phosphatase inhibitors, MCE) containing magnetic beads conjugated to specific antibodies (anti-IGF2BP2, anti-METTL16, anti-m 6 A).

Techniques: Staining, RNA Immunoprecipitation, Immunoprecipitation, Over Expression

Journal: Experimental and Therapeutic Medicine

Article Title: Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells

doi: 10.3892/etm.2018.7132

Figure Lengend Snippet: IGF-2 stimulated the proliferation of HemSCs in vitro . (A) IGF-2 promoted the proliferation of HemSCs in vitro ; IGF-2 at 100 and 200 ng/ml significantly promoted the proliferation of HemSCs as compared with the control group, and IGF-2 at 100 ng/ml exhibited the highest proliferation activity among all groups. (B) Growth curves of IGF-2 treated HemSCs. The proliferation of IGF-2-treated HemSCs (days 1–7) was promoted compared with the untreated group. Values are mean ± SD, n =3; *P<0.05 and **P<0.01 vs. Control group; # P<0.05 vs. 100 ng/ml. IGF-2, insulin-like growth factor-2; HemSC, hemangioma stem cell.

Article Snippet: The cells were cultured in EGM-2/FBS-10% containing IGF-2 (100 ng/ml), IGF-2 (100 ng/ml) plus OSI-906 (1 μM), IGF-2 (100 ng/ml) plus LY294002 (cat. no. HY-10108; 10 μM; MedChem Express Co., Ltd., Shanghai, China), or no treatment.

Techniques: In Vitro, Control, Activity Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells

doi: 10.3892/etm.2018.7132

Figure Lengend Snippet: Expressions of IGF-2 and its receptors in HemSCs and the effect of IGF-2 on the adipogenesis of HemSCs. (A) Immunohistochemistry of HemSCs using antibodies specific to IGF-2, IGF-2R, IGF-1R, and IR. (B) IGF-2 induced differentiation of HemSCs into adipocytes. HemSCs that were cultivated for 10 days in adipogenic differentiation media; adipogenic differentiation media with IGF-2 (100 ng/ml); adipogenic differentiation media with IGF-2 (100 ng/ml) plus OSI-906 (1 µM). After Oil Red O-staining, phase-contrast microscopy was used to observe lipid droplets. Scale bar, 100 µm. IGF-2, insulin-like growth factor-2; HemSC, hemangioma stem cell; IGF-1R, IGF-1 receptor; IR, insulin receptor.

Article Snippet: The cells were cultured in EGM-2/FBS-10% containing IGF-2 (100 ng/ml), IGF-2 (100 ng/ml) plus OSI-906 (1 μM), IGF-2 (100 ng/ml) plus LY294002 (cat. no. HY-10108; 10 μM; MedChem Express Co., Ltd., Shanghai, China), or no treatment.

Techniques: Immunohistochemistry, Staining, Microscopy

Journal: Experimental and Therapeutic Medicine

Article Title: Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells

doi: 10.3892/etm.2018.7132

Figure Lengend Snippet: Effects of IGF-2 on the expression of adipogenic markers in HemSCs. The C/EBPα, C/EBPβ, PPAR, and adiponectin protein levels were analyzed by western blot analysis. (A) The results showing protein levels in untreated and treated HemSCs for C/EBPα, C/EBPβ, PPARγ, and adiponectin in IGF-2 (100 ng/ml), IGF-2 (100 ng/ml) with OSI-906 (1 µM), IGF-2 (100 ng/ml) with LY294002 (10 µM), and LY294002 (10 µM). β-actin was used as a loading control. (B) Western blot analysis showed p-AKT and total AKT as well as β-actin protein bands in confluent HemSCs cultures exposed to the indicated treatments for 1 h. Groups: No treatment; IGF-2 (100 ng/ml); IGF-2 (100 ng/ml) plus OSI-906 (1 µM); IGF-2 (100 ng/ml) plus LY294002 (10 µM). Quantification of the p-AKT protein levels showed an obvious increase in the IGF-2 group (P<0.05). No changes were detected in the total AKT protein levels among the groups (P>0.05). Values are mean ± SD. n =3, *P<0.05 vs. Control group. # P<0.05 vs. IGF-2. IGF-2, insulin-like growth factor-2; HemSC, hemangioma stem cell; p-AKT, phosphorylated AKT.

Article Snippet: The cells were cultured in EGM-2/FBS-10% containing IGF-2 (100 ng/ml), IGF-2 (100 ng/ml) plus OSI-906 (1 μM), IGF-2 (100 ng/ml) plus LY294002 (cat. no. HY-10108; 10 μM; MedChem Express Co., Ltd., Shanghai, China), or no treatment.

Techniques: Expressing, Western Blot, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells

doi: 10.3892/etm.2018.7132

Figure Lengend Snippet: Analysis of the adipogenic gene and protein expression of propranolol-treated HemSCs. (A) Western blot analysis results of protein levels in propranolol-treated HemSCs for C/EBPα, C/EBPβ, and PPAR in 0, 50, and 100 µM. (B) Quantitative RT-PCR was performed to analyze the expression of C/EBPα, C/EBPβ, and PPAR. (C) Western blot analysis showed IGF-2 and β-actin protein bands in propranolol-treated HemSCs. Values are mean ± SD. n =3; **P<0.01 vs. Control group. HemSC, hemangioma stem cell; IGF-2, insulin-like growth factor-2.

Article Snippet: The cells were cultured in EGM-2/FBS-10% containing IGF-2 (100 ng/ml), IGF-2 (100 ng/ml) plus OSI-906 (1 μM), IGF-2 (100 ng/ml) plus LY294002 (cat. no. HY-10108; 10 μM; MedChem Express Co., Ltd., Shanghai, China), or no treatment.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Control

Journal: Frontiers in Endocrinology

Article Title: Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin

doi: 10.3389/fendo.2018.00131

Figure Lengend Snippet: Serum levels of (A) Gh, (B) estradiol, (C) Igf1, (D) Igf2, and (E) glucose contents in female (F) and male (M) non-transgenic (NT) and GH-transgenic (T) common carp. The results obtained were analyzed within sex by independent sample t -test. The P -values are indicated on the graph.

Article Snippet: Igf1 (CSB-E12122Fh) and Igf2 (CSB-EL011088FI) ELISA kits were purchased from Cusabio Life Science (Wuhan, China) ( ).

Techniques: Transgenic Assay